BAIT

AS2

ASYMMETRIC LEAVES 2, F5I14.15, F5I14_15, AT1G65620
protein ASYMMETRIC LEAVES 2
GO Process (5)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

EMF2

AT5G51240, AtEMF2, CYR1, CYTOKININ RESISTANT 1, EMBRYONIC FLOWER 2, MWD22.18, MWD22_18, VEF2, AT5G51230
polycomb group protein EMBRYONIC FLOWER 2
GO Process (2)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The ASYMMETRIC LEAVES complex maintains repression of KNOX homeobox genes via direct recruitment of Polycomb-repressive complex2.

Lodha M, Marco CF, Timmermans MC

Polycomb-repressive complexes (PRCs) ensure the correct spatiotemporal expression of numerous key developmental regulators. Despite their pivotal role, how PRCs are recruited to specific targets remains largely unsolved, particularly in plants. Here we show that the Arabidopsis ASYMMETRIC LEAVES complex physically interacts with PRC2 and recruits this complex to the homeobox genes BREVIPEDICELLUS and KNAT2 to stably silence these stem cell ... [more]

Genes Dev. Mar. 06, 2013; 0(0); [Pubmed: 23468429]

Throughput

  • Low Throughput

Curated By

  • BioGRID