BAIT

IAA8

T9I22.11, T9I22_11, indoleacetic acid-induced protein 8, AT2G22670
auxin-responsive protein IAA8
GO Process (3)
GO Function (2)
GO Component (2)
Arabidopsis thaliana (Columbia)
PREY

MP

ARF5, AUXIN RESPONSE FACTOR 5, F6F9.10, F6F9_10, IAA24, MONOPTEROS, TRANSCRIPTION FACTOR IAA24, indole-3-acetic acid inducible 24, AT1G19850
auxin response factor 5
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A gain-of-function mutation in IAA8 alters Arabidopsis floral organ development by change of jasmonic acid level.

Wang J, Yan DW, Yuan TT, Gao X, Lu YT

Auxin regulates a variety of physiological processes via its downstream factors included Aux/IAAs. In this study, one of these Aux/IAAs, IAA8 is shown to play its role in Arabidopsis development with transgenic plants expressing GFP-mIAA8 under the control of IAA8 promoter, in which IAA8 protein was mutated by changing Pro170 to Leu170 in its conserved domain II. These transgenic dwarfed ... [more]

Plant Mol. Biol. Mar. 13, 2013; 0(0); [Pubmed: 23483289]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IAA8 MP
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Low-BioGRID
-
IAA8 MP
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-
MP IAA8
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-
MP IAA8
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID