BAIT

WEE1

ATWEE1, F22D16.3, F22D16_3, WEE1 kinase homolog, WEE1-KINASE, AT1G02970
Wee1-like protein kinase
GO Process (2)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

SKIP1

MTI20.16, MTI20_16, SKP1 interacting partner 1, AT5G57900
F-box protein SKIP1
GO Process (0)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Plant WEE1 kinase is cell cycle regulated and removed at mitosis via the 26S proteasome machinery.

Cook GS, Lentz Gronlund A, Siciliano I, Spadafora N, Amini M, Herbert RJ, Bitonti MB, Graumann K, Francis D, Rogers HJ

In yeasts and animals, premature entry into mitosis is prevented by the inhibitory phosphorylation of cyclin-dependent kinase (CDK) by WEE1 kinase, and, at mitosis, WEE1 protein is removed through the action of the 26S proteasome. Although in higher plants WEE1 function has been confirmed in the DNA replication checkpoint, Arabidopsis wee1 insertion mutants grow normally, and a role for the ... [more]

J. Exp. Bot. Mar. 27, 2013; 0(0); [Pubmed: 23536609]

Throughput

  • Low Throughput

Curated By

  • BioGRID