Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Allosteric Regulation of Transport Activity by Heterotrimerization of Arabidopsis Ammonium Transporter Complexes in Vivo.

Yuan L, Gu R, Xuan Y, Smith-Valle E, Loque D, Frommer WB, von Wiren N

Ammonium acquisition by plant roots is mediated by AMMONIUM TRANSPORTERs (AMTs), ubiquitous membrane proteins with essential roles in nitrogen nutrition in all organisms. In microbial and plant cells, ammonium transport activity is controlled by ammonium-triggered feedback inhibition to prevent cellular ammonium toxicity. Data from heterologous expression in yeast indicate that oligomerization of plant AMTs is critical for allosteric regulation of ... [more]

Plant Cell Mar. 05, 2013; 0(0); [Pubmed: 23463773]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AMT1;3 AMT1;1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Bellati J (2016)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AMT1;1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ammonium transmembrane transporter activity [IDA, IMP, ISS]

Gene Ontology Cellular Component

AMT1;3