BAIT

AT1G49230

F27J15.2, F27J15_2
RING-H2 finger protein ATL78
GO Process (0)
GO Function (0)
GO Component (0)
Arabidopsis thaliana (Columbia)
PREY

UBC10

ubiquitin-conjugating enzyme 10, AT5G53300
ubiquitin-conjugating enzyme E2 10
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Suppression of Arabidopsis RING E3 ubiquitin ligase AtATL78 increases tolerance to cold stress and decreases tolerance to drought stress.

Kim SJ, Kim WT

AtATL78 is an Arabidopsis RING E3 ubiquitin ligase. RT-PCR and promoter-GUS assays revealed that AtATL78 was up-regulated by cold stress and down-regulated by drought. AtATL78 was localized at the plasma-membrane. Suppression of AtATL78 increased tolerance to cold stress but decreased tolerance to drought. Our data suggests that AtATL78 is a negative regulator of cold stress response and a positive regulator ... [more]

FEBS Lett. Jul. 03, 2013; 0(0); [Pubmed: 23831064]

Throughput

  • Low Throughput

Additional Notes

  • E1 (His-UBA1)
  • E2 (His-UBC8 or His-UBC10)

Curated By

  • BioGRID