Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Interactions and CCAAT-Binding of Arabidopsis thaliana NF-Y Subunits.

Calvenzani V, Testoni B, Gusmaroli G, Lorenzo M, Gnesutta N, Petroni K, Mantovani R, Tonelli C

NF-Y is a transcription factor that recognizes with high specificity and affinity the widespread CCAAT box promoter element. It is formed by three subunits: NF-YA and the NF-YB/NF-YC- heterodimer containing histone fold domains (HFDs). We previously identified a large NF-Y gene family in Arabidopsis thaliana, composed of 29 members, and characterized their expression patterns in various plant tissues.We used yeast ... [more]

PLoS ONE Aug. 23, 2012; 7(8);e42902 [Pubmed: 22912760]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NF-YC9 NF-YB7	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Calvenzani V (2012)	High	-	BioGRID	-
NF-YB7 NF-YC9	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hackenberg D (2011)	Low	-	BioGRID	-
NF-YC9 NF-YB7	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hackenberg D (2011)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

NF-YB7

Gene Ontology Molecular Function

sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

NF-YC9

Gene Ontology Biological Process