DCL1
Gene Ontology Biological Process
- RNA processing [ISS]
- cytokinesis by cell plate formation [IMP]
- embryonic pattern specification [IMP]
- flower development [TAS]
- mRNA cleavage involved in gene silencing by miRNA [IMP]
- primary miRNA processing [TAS]
- production of lsiRNA involved in RNA interference [IMP]
- production of miRNAs involved in gene silencing by miRNA [IGI]
- production of siRNA involved in RNA interference [IMP]
- production of ta-siRNAs involved in RNA interference [IMP]
- regulation of seed maturation [IMP]
- seed morphogenesis [IMP]
- suspensor development [IMP]
- vegetative to reproductive phase transition of meristem [IMP]
- virus induced gene silencing [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SE
Gene Ontology Biological Process
- RNA splicing [IMP]
- chromatin modification [TAS]
- primary miRNA processing [IMP]
- production of ta-siRNAs involved in RNA interference [IMP]
- regulation of adaxial/abaxial pattern formation [IMP]
- regulation of meristem development [IMP]
- regulation of transcription, DNA-templated [TAS]
- shoot system development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
Complementation of Hyponastic Leaves1 by Double-strand RNA Binding Domains of Dicer-like 1 in Nuclear Dicing Bodies.
MiRNAs are a class of small, regulatory RNAs that are found in almost all of the eukaryotes. Arabidopsis (Arabidopsis thaliana) miRNAs are processed from primary microRNAs (pri-miRNAs) mainly by the RNase III-like enzyme DICER-LIKE1 (DCL1) and its specific partner HYPONASTIC LEAVES1 (HYL1), a double-strand RNA-binding protein, both contain two double-strand RNA-binding domains (dsRBDs). These dsRBDs are essential for miRNA processing, ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SE DCL1 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | 281038 | |
| SE DCL1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID