BAIT

RBOH F

ARABIDOPSIS THALIANA RESPIRATORY BURST OXIDASE HOMOLOG F, ATRBOH F, ATRBOHF, CYTOCHROME B245 BETA CHAIN HOMOLOG RBOHAP108, F22C12.18, F22C12_18, RBOHAP108, RBOHF, RESPIRATORY BURST OXIDASE PROTEIN F, AT1G64060
respiratory burst oxidase
Arabidopsis thaliana (Columbia)
PREY

SRC2

(AT)SRC2, F7G19.6, F7G19_6, SOYBEAN GENE REGULATED BY COLD-2, AT1G09070
protein SRC2
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A low temperature-inducible protein AtSRC2 enhances the ROS-producing activity of NADPH oxidase AtRbohF.

Kawarazaki T, Kimura S, Iizuka A, Hanamata S, Nibori H, Michikawa M, Imai A, Abe M, Kaya H, Kuchitsu K

Reactive oxygen species (ROS) produced by NADPH oxidases play critical roles in plant environmental responses. Arabidopsis thaliana NADPH oxidase AtRbohF-mediated ROS-production is involved in abiotic stress responses. Because overproduction of ROS is highly toxic to cells, the activity of AtRbohF needs to be tightly regulated in response to diverse stimuli. The ROS-producing activity of AtRbohF is activated by Ca(2+) and ... [more]

Biochim. Biophys. Acta Jul. 16, 2013; 0(0); [Pubmed: 23872431]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RBOH F SRC2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID