A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Functional role of oligomerization for bacterial and plant SWEET sugar transporter family.

Xuan YH, Hu YB, Chen LQ, Sosso D, Ducat DC, Hou BH, Frommer WB

Eukaryotic sugar transporters of the MFS and SWEET superfamilies consist of 12 and 7 Î±-helical transmembrane domains (TMs), respectively. Structural analyses indicate that MFS transporters evolved from a series of tandem duplications of an ancestral 3-TM unit. SWEETs are heptahelical proteins carrying a tandem repeat of 3-TM separated by a single TM. Here, we show that prokaryotes have ancestral SWEET ... [more]

Proc. Natl. Acad. Sci. U.S.A. Sep. 11, 2013; 0(0); [Pubmed: 24027245]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
AT4G15920 AT1G21460	PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.	Xuan YH (2013)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

AT1G21460

Gene Ontology Molecular Function

sugar transmembrane transporter activity [IDA]

Gene Ontology Cellular Component

AT4G15920

Gene Ontology Biological Process