Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates auxin response in Arabidopsis thaliana.

Li S, Zachgo S

TCP proteins belong to the plant-specific bHLH transcription factor family and function as key regulators of diverse developmental processes. Functional redundancy amongst family members and posttranscriptional downregulation by miRJAW of several TCP genes complicate their functional characterization. Here, we explored the role of TCP3 by analyzing transgenic plants expressing the miRJAW-resistant mTCP3 and the dominant-negative TCP3SRDX. Seedlings and seeds of ... [more]

Plant J. Oct. 12, 2013; 0(0); [Pubmed: 24118612]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TCP3 MYB90	PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.	Li S (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

TCP3

Gene Ontology Biological Process

Gene Ontology Molecular Function

sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

MYB90