BAIT

AT4G27420

F27G19.20, F27G19_20
ABC transporter G family member 9
GO Process (2)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)
PREY

WBC11

ABCG11, ARABIDOPSIS THALIANA WHITE-BROWN COMPLEX HOMOLOG PROTEIN 11, ATWBC11, COF1, CUTICULAR DEFECT AND ORGAN FUSION 1, DESPERADO, DSO, F2H15.7, F2H15_7, white-brown complex homolog protein 11, AT1G17840
ABC transporter G family member 11
Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis.

Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C

In order to gain insights into the regulatory pathways controlling phloem development, we characterized three genes encoding membrane proteins from the G subfamily of ABC transporters (ABCG9, ABCG11 and ABCG14), whose expressions in the phloem were confirmed. Mutations in the genes encoding these dimerizing "half transporters" are semidominant and result in vascular patterning defects in cotyledons and floral stem. Coimmunoprecipitation ... [more]

Plant J. Sep. 24, 2013; 0(0); [Pubmed: 24112720]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
AT4G27420 WBC11
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
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Curated By

  • BioGRID