BAIT

PRK2A

ATPRK2A, T4E14.15, T4E14_15, AT2G07040
pollen receptor kinase 2A
Arabidopsis thaliana (Columbia)
PREY

ROPGEF12

ATROPGEF12, F19K16.18, F19K16_18, KINASE PARTNER PROTEIN-LIKE, KPP-LIKE, MATERNAL EFFECT EMBRYO ARREST 64, MEE64, RHO guanyl-nucleotide exchange factor 12, AT1G79860
RHO guanyl-nucleotide exchange factor 12
GO Process (3)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The juxtamembrane and carboxy-terminal domains of Arabidopsis PRK2 are critical for ROP-induced growth in pollen tubes.

Zhao XY, Wang Q, Li S, Ge FR, Zhou LZ, McCormick S, Zhang Y

Polarized growth of pollen tubes is a critical step for successful reproduction in angiosperms and is controlled by ROP GTPases. Spatiotemporal activation of ROP (Rho GTPases of plants) necessitates a complex and sophisticated regulatory system, in which guanine nucleotide exchange factors (RopGEFs) are key components. It was previously shown that a leucine-rich repeat receptor-like kinase, Arabidopsis pollen receptor kinase 2 ... [more]

J. Exp. Bot. Oct. 17, 2013; 0(0); [Pubmed: 24136420]

Throughput

  • Low Throughput

Curated By

  • BioGRID