A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Monothiol glutaredoxin-BolA interactions: redox control of Arabidopsis thaliana BolA2 and SufE1.

Couturier J, Wu HC, Dhalleine T, Pegeot H, Sudre D, Gualberto J, Jacquot JP, Gaymard F, Vignols F, Rouhier N

A functional relationship between monothiol glutaredoxins and BolA has been unravelled by genomic analyses and in several high-throughput studies. Phylogenetic analyses coupled to transient expression of GFP fusions indicated that, in addition to the sulfurtransferase SufE1, which contains a C-terminal BolA domain, three BolA isoforms exist in Arabidopsis thaliana, BolA1 being plastidial, BolA2 nucleo-cytoplasmic and BolA4 dual-targeted to mitochondria and ... [more]

Mol Plant Nov. 07, 2013; 0(0); [Pubmed: 24203231]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CXIP2 AT1G55805	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Couturier J (2013)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CXIP2

Gene Ontology Biological Process

Gene Ontology Cellular Component

AT1G55805

Gene Ontology Molecular Function

protein binding [IPI]