BAIT
FBXO6
FBG2, FBS2, FBX6, Fbx6b, RP3-330O12.3
F-box protein 6
GO Process (5)
GO Function (2)
GO Component (2)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RAB27A
GS2, HsT18676, RAB27, RAM
RAB27A, member RAS oncogene family
GO Process (21)
GO Function (4)
GO Component (8)
Gene Ontology Biological Process
- GTP catabolic process [IBA]
- Rab protein signal transduction [IBA]
- antigen processing and presentation [IMP]
- cellular protein metabolic process [TAS]
- complement-dependent cytotoxicity [IMP]
- exocytosis [IDA]
- exosomal secretion [IMP]
- intracellular protein transport [IBA]
- melanosome localization [IMP]
- melanosome transport [IBA]
- multivesicular body organization [IMP]
- multivesicular body sorting pathway [IMP]
- positive regulation of constitutive secretory pathway [IMP]
- positive regulation of exocytosis [IMP]
- positive regulation of gene expression [IMP]
- positive regulation of phagocytosis [IMP]
- positive regulation of reactive oxygen species biosynthetic process [IMP]
- positive regulation of regulated secretory pathway [IGI, IMP]
- protein secretion [IBA]
- synaptic vesicle transport [TAS]
- vesicle docking involved in exocytosis [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Proteomic identification of common SCF ubiquitin ligase FBXO6-interacting glycoproteins in three kinds of cells.
FBOX6 ubiquitin ligase complex is involved in the endoplasmic reticulum-associated degradation pathway by mediating the ubiquitination of glycoproteins. FBXO6 interacts with the chitobiose in unfolded N-glycoprotein, pointing glycoproteins toward E2 for ubiquitination. Although the glycoprotein-recognizing mechanism of FBXO6 is well documented, its bona fide interacting glycoproteins are largely unknown. Here we utilized a protein purification approach combined with LC-MS to ... [more]
J. Proteome Res. Mar. 02, 2012; 11(3);1773-81 [Pubmed: 22268729]
Throughput
- High Throughput
Ontology Terms
- disease: t-cell leukemia (DOID:715)
- cell line: jurkat cell (BTO:0000661)
Additional Notes
- exogenous expression of bait
Curated By
- BioGRID