Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Identification of Arabidopsis SUMO-interacting proteins that regulate chromatin activity and developmental transitions.

Elrouby N, Bonequi MV, Porri A, Coupland G

Posttranslational modification of proteins by small ubiquitin-like modifier (SUMO) plays essential roles in eukaryotic growth and development. Many covalently modified SUMO targets have been identified; however, the extent and significance of noncovalent interactions of SUMO with cellular proteins is poorly understood. Here, large-scale yeast two-hybrid screens repeatedly identified a surprisingly small number of proteins that interacted with three Arabidopsis SUMO ... [more]

Proc. Natl. Acad. Sci. U.S.A. Nov. 19, 2013; 0(0); [Pubmed: 24255109]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SUMO2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein tag [IDA]

Gene Ontology Cellular Component

MOM