DCAF1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
ABI5
Gene Ontology Biological Process
- negative regulation of seed germination [IMP]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of transcription, DNA-templated [IMP, TAS]
- response to abscisic acid [IEP, IMP]
- response to chitin [IEP]
- response to gibberellin [IEP]
- response to salt stress [IEP]
- response to water deprivation [IEP]
- seed development [IMP]
- seed germination [IEP]
- sugar mediated signaling pathway [TAS]
Gene Ontology Molecular Function
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
ABD1 Is an Arabidopsis DCAF Substrate Receptor for CUL4-DDB1-Based E3 Ligases That Acts as a Negative Regulator of Abscisic Acid Signaling.
Members of the DDB1-CUL4-associated factors (DCAFs) family directly bind to DAMAGED DNA BINDING PROTEIN1 (DDB1) and function as the substrate receptors in CULLIN4-based E3 (CUL4) ubiquitin ligases, which regulate the selective ubiquitination of proteins. Here, we describe a DCAF protein, ABD1 (for ABA-hypersensitive DCAF1), that negatively regulates abscisic acid (ABA) signaling in Arabidopsis thaliana. ABD1 interacts with DDB1 in vitro ... [more]
Throughput
- Low Throughput
Additional Notes
- luciferase complementation assay
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| DCAF1 ABI5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 935667 | |
| DCAF1 ABI5 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID