BAIT

PGGT-I

ATGGT-IB, GERANYLGERANYLTRANSFERASE-I BETA SUBUNIT, GGB, AT2G39550
geranylgeranyl transferase type-1 subunit beta
Arabidopsis thaliana (Columbia)
PREY

FTA

ATFTA, FARNESYLTRANSFERASE A, FARNESYLTRANSFERASE SUBUNIT A, PFT/PGGT-IALPHA, PLP, PLURIPETALA, AT3G59380
farnesyltransferase A
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A mesoscale abscisic acid hormone interactome reveals a dynamic signaling landscape in Arabidopsis.

Lumba S, Toh S, Handfield LF, Swan M, Liu R, Youn JY, Cutler SR, Subramaniam R, Provart N, Moses A, Desveaux D, McCourt P

The sesquiterpenoid abscisic acid (ABA) mediates an assortment of responses across a variety of kingdoms including both higher plants and animals. In plants, where most is known, a linear core ABA signaling pathway has been identified. However, the complexity of ABA-dependent gene expression suggests that ABA functions through an intricate network. Here, using systems biology approaches that focused on genes ... [more]

Dev. Cell May. 12, 2014; 29(3);360-72 [Pubmed: 24823379]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PGGT-I FTA
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID