Transcription coactivator SAYP combines chromatin remodeler Brahma and transcription initiation factor TFIID into a single supercomplex.
Transcription activation by RNA polymerase II is a complicated process driven by combined, precisely coordinated action of a wide array of coactivator complexes, which carry out chromatin-directed activities and nucleate the assembly of the preinitiation complex on the promoter. Using various techniques, we have shown the existence of a stable ... coactivator supercomplex consisting of the chromatin-remodeling factor Brahma (SWI/SNF) and the transcription initiation factor TFIID, named BTFly (Brahma and TFIID in one assembly). The coupling of Brahma and TFIID is mediated by the SAYP factor, whose evolutionarily conserved activation domain SAY can directly bind to both BAP170 subunit of Brahma and TAF5 subunit of TFIID. The integrity of BTFly is crucial for its ability to activate transcription. BTFly is distributed genome-wide and appears to be a means of effective transcription activation.
Mesh Terms:
Animals, Cell Cycle Proteins, Cell Line, Chromatin Assembly and Disassembly, Drosophila Proteins, Drosophila melanogaster, Embryo, Nonmammalian, Molecular Weight, Multiprotein Complexes, Protein Binding, Protein Structure, Tertiary, Trans-Activators, Transcription Factor TFIID, Transcription Factors, Transcriptional Activation
Animals, Cell Cycle Proteins, Cell Line, Chromatin Assembly and Disassembly, Drosophila Proteins, Drosophila melanogaster, Embryo, Nonmammalian, Molecular Weight, Multiprotein Complexes, Protein Binding, Protein Structure, Tertiary, Trans-Activators, Transcription Factor TFIID, Transcription Factors, Transcriptional Activation
Proc. Natl. Acad. Sci. U.S.A.
Date: Jul. 07, 2009
PubMed ID: 19541607
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