Antagonistic roles of Drosophila Tctp and Brahma in chromatin remodelling and stabilizing repeated sequences.

Genome stability is essential for all organisms. Translationally controlled tumour protein (TCTP) is a conserved protein associated with cancers. TCTP is involved in multiple intracellular functions, but its role in transcription and genome stability is poorly understood. Here, we demonstrate new functions of Drosophila TCTP (Tctp) in transcription and the ...
stability of repeated sequences (rDNA and pericentromeric heterochromatin). Tctp binds Brahma (Brm) chromatin remodeler to negatively modulate its activity. Tctp mutants show abnormally high levels of transcription in a large set of genes and transposons. These defects are ameliorated by brm mutations. Furthermore, Tctp promotes the stability of repeated sequences by opposing the Brm function. Additional regulation of pericentromeric heterochromatin by Tctp is mediated by su(var)3-9 transcriptional regulation. Altogether, Tctp regulates transcription and the stability of repeated sequences by antagonizing excess Brm activity. This study provides insights into broader nuclear TCTP functions for the maintenance of genome stability.
Mesh Terms:
Animals, Argonaute Proteins, Chromatin, Drosophila Proteins, Drosophila melanogaster, Gene Expression Regulation, HSP70 Heat-Shock Proteins, Heat-Shock Response, MicroRNAs, Promoter Regions, Genetic, Protein Binding, RNA Helicases, RNA Interference, RNA Polymerase II, RNA, Double-Stranded, RNA-Binding Proteins, Ribonuclease III, Transcription Factors, Transcription, Genetic
Nat Commun
Date: Sep. 30, 2016
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