BAIT

MAD2

SPBC20F10.06
mitotic spindle checkpoint protein Mad2
Schizosaccharomyces pombe (972h)
PREY

POT1

SPAC26H5.06
telomere end-binding protein Pot1
GO Process (3)
GO Function (3)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Rad51-dependent aberrant chromosome structures at telomeres and ribosomal DNA activate the spindle assembly checkpoint.

Nakano A, Masuda K, Hiromoto T, Takahashi K, Matsumoto Y, Habib AG, Darwish AG, Yukawa M, Tsuchiya E, Ueno M

The spindle assembly checkpoint (SAC) monitors defects in kinetochore-microtubule attachment or lack of tension at kinetochores and arrests cells at prometaphase. In fission yeast, the double mutant between pot1Δ and the helicase-dead point mutant of the RecQ helicase Rqh1 gene (rqh1-hd) accumulates Rad51-dependent recombination intermediates at telomeres and enters mitosis with those intermediates. Here, we found that SAC-dependent prometaphase arrest ... [more]

Mol. Cell. Biol. Apr. 01, 2014; 34(8);1389-97 [Pubmed: 24469396]

Throughput

  • Low Throughput

Ontology Terms

  • vegetative growth (APO:0000106)
  • resistance to chemicals (APO:0000087)

Additional Notes

  • Figure 3
  • mad2 pot1 double mutant has increased sensitivity to TBZ (rqh1-hd background)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
POT1 MAD2
Phenotypic Suppression
Phenotypic Suppression

A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
1026602

Curated By

  • BioGRID