BAIT
SEMA-1A
BcDNA:RE36155, CG18405, CT26404, D-Sema 1a, D-Sema I, D-Sema-I, D-SemaI, Dmel\CG18405, Dsema 1a, Dsema-I, Fas-IV, FasIV, Sema 1a, Sema-I, Sema1A, SemaI, l(2)k13702, sema, Dmel_CG18405
CG18405 gene product from transcript CG18405-RE
GO Process (11)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- axon guidance [IDA, IMP, ISS, NAS]
- axon midline choice point recognition [IMP]
- brain morphogenesis [IMP]
- dendrite morphogenesis [IMP]
- locomotion involved in locomotory behavior [IMP]
- motor neuron axon guidance [IMP]
- negative regulation of cell size [IMP]
- semaphorin-plexin signaling pathway involved in regulation of photoreceptor cell axon guidance [IGI]
- startle response [IMP]
- synapse assembly [IMP]
- synaptic target recognition [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Drosophila melanogaster
PREY
TOR
5092, CG5092, CT16317, CT24745, CT24817, DmTOR, Dmel\CG5092, FRAP/TOR, dTOR, l(2)k17004, mTOR, Dmel_CG5092
Target of rapamycin
GO Process (25)
GO Function (2)
GO Component (0)
Gene Ontology Biological Process
- axon guidance [IGI]
- cellular response to DNA damage stimulus [IMP]
- dendrite morphogenesis [IMP]
- detection of nutrient [TAS]
- determination of adult lifespan [IMP]
- endocytic recycling [IDA]
- gonad development [IMP]
- growth [TAS]
- multicellular organism growth [IMP]
- myoblast fusion [IMP]
- negative regulation of macroautophagy [IMP]
- phosphorylation [NAS]
- phototaxis [IGI]
- positive regulation of cell growth [NAS, TAS]
- positive regulation of cell size [IGI, IMP, TAS]
- positive regulation of multicellular organism growth [TAS]
- positive regulation of organ growth [TAS]
- positive regulation of protein phosphorylation [IGI]
- positive regulation of ribosome biogenesis [IMP]
- regulation of cell growth [IDA]
- regulation of reactive oxygen species metabolic process [IGI]
- regulation of response to drug [IMP]
- response to nutrient [TAS]
- somatic muscle development [IMP]
- synaptic growth at neuromuscular junction [IGI]
Gene Ontology Molecular Function
Drosophila melanogaster
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
In vivo analysis of proteomes and interactomes using Parallel Affinity Capture (iPAC) coupled to mass spectrometry.
Affinity purification coupled to mass spectrometry provides a reliable method for identifying proteins and their binding partners. In this study we have used Drosophila melanogaster proteins triple tagged with Flag, Strep II, and Yellow fluorescent protein in vivo within affinity pull-down experiments and isolated these proteins in their native complexes from embryos. We describe a pipeline for determining interactomes by ... [more]
Mol. Cell Proteomics Jun. 01, 2011; 10(6);M110.002386 [Pubmed: 21447707]
Throughput
- High Throughput
Curated By
- BioGRID