BAIT

GAG

HIV1gp2
Pr55(Gag)
GO Process (0)
GO Function (0)
GO Component (0)
Human Immunodeficiency Virus 1
PREY

TPR

translocated promoter region, nuclear basket protein
GO Process (37)
GO Function (12)
GO Component (13)

Gene Ontology Biological Process

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Analysis of HIV-1 Gag protein interactions via biotin ligase tagging.

Ritchie C, Cylinder I, Platt EJ, Barklis E

We have examined the interactions of wild-type (WT) and matrix protein-deleted (ΔMA) HIV-1 precursor Gag (PrGag) proteins in virus-producing cells using a biotin ligase-tagging approach. To do so, WT and ΔMA PrGag proteins were tagged with the Escherichia coli promiscuous biotin ligase (BirA*), expressed in cells, and examined. Localization patterns of PrGag proteins and biotinylated proteins overlapped, consistent with observations ... [more]

J. Virol. Apr. 01, 2015; 89(7);3988-4001 [Pubmed: 25631074]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • P04591
  • PRO_0000261216
  • P04591#PRO_0000261216
  • NP_057850.1

Curated By

  • BioGRID