BAIT

CLEC-63

CELE_F35C5.6, F35C5.6

C-type LECtin

GO Process (0)

GO Function (0)

GO Component (0)

WormBase Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Caenorhabditis elegans

PREY

LYS-1

CELE_Y22F5A.4, Y22F5A.4

lys-1 encodes a putative lysozyme, whose overexpression increases resistance to infection by Serratia marcescens; lys-1(RNAi) animals have normal resistance to S. marcescens, perhaps because of genetic redundancy with the LYS-1 paralogs LYS-2 to -8 and LYS-10; in uninfected animals, LYS-1 is expressed in the intestine and in IL1/IL2 neurons, along with a few unidentified head neurons; in intestinal cells, LYS-1 localizes to apical vesicles; LYS-1 expression is induced by infection by Pseudomonas aeruginosa but not S. marcescens; LYS-1 expression is promoted by DBL-1, TIR-1 and NSY-1, and perhaps also by ELT-2, but not by DAF-16; LYS-1 and its paralogs are more similar to lysozymes from the amoeboid protozoon Entamoeba histolytica than to more familiar ones from vertebrates.

GO Process (3)

GO Function (0)

GO Component (2)

Gene Ontology Biological Process

defense response to Gram-negative bacterium [IEP]

defense response to Gram-positive bacterium [IMP]

innate immune response [IEP, IMP]

Gene Ontology Cellular Component

apical part of cell [IDA]

cytoplasmic vesicle [IDA]

WormBase Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Caenorhabditis elegans

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

EPIC: software toolkit for elution profile-based inference of protein complexes.

Hu LZ, Goebels F, Tan JH, Wolf E, Kuzmanov U, Wan C, Phanse S, Xu C, Schertzberg M, Fraser AG, Bader GD, Emili A

Protein complexes are key macromolecular machines of the cell, but their description remains incomplete. We and others previously reported an experimental strategy for global characterization of native protein assemblies based on chromatographic fractionation of biological extracts coupled to precision mass spectrometry analysis (chromatographic fractionation-mass spectrometry, CF-MS), but the resulting data are challenging to process and interpret. Here, we describe EPIC ... [more]

Nat. Methods Jul. 15, 2019; (); [Pubmed: 31308550]

Quantitative Score

0.582 [Confidence Score]

Throughput

High Throughput

Additional Notes

High confidence protein interactions have an EPIC PPI score >= 0.5

Curated By

BioGRID

Interaction Summary