BAIT

CLEC-63

CELE_F35C5.6, F35C5.6
C-type LECtin
GO Process (0)
GO Function (0)
GO Component (0)
Caenorhabditis elegans
PREY

HSP-6

CELE_C37H5.8, mot-2, C37H5.8
hsp-6 encodes a nuclear-encoded mitochondrion-specific chaperone that is a member of the DnaK/Hsp70 superfamily of molecular chaperones; hsp-6 is involved in the mitochondrial unfolded protein response, as hsp-6 expression, normally broadly detected from the L1 larval through adult stages of development, is greatly increased in response to treatments that disrupt mitochondrial protein handling; loss of hsp-6 activity via RNAi results in severe growth defects with arrest at embryonic and early larval stages of development; loss of hsp-6 via RNAi in young adult hermaphrodites results in abnormal mitochondrial membrane potential and morphology, accompanied by reduction of ATP levels and reduced levels of mitochondrial proteins ATP-2, HSP-60, and CLK-1; in addition, hsp-6(RNAi) of adults disrupts gonadal function, motility, and pharyngeal pumping, as well as resulting in reduced lifespan; an HSP-6::GFP fusion protein localizes to mitochondria; HSP-6 levels are markedly reduced in aged worms.
Caenorhabditis elegans

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

EPIC: software toolkit for elution profile-based inference of protein complexes.

Hu LZ, Goebels F, Tan JH, Wolf E, Kuzmanov U, Wan C, Phanse S, Xu C, Schertzberg M, Fraser AG, Bader GD, Emili A

Protein complexes are key macromolecular machines of the cell, but their description remains incomplete. We and others previously reported an experimental strategy for global characterization of native protein assemblies based on chromatographic fractionation of biological extracts coupled to precision mass spectrometry analysis (chromatographic fractionation-mass spectrometry, CF-MS), but the resulting data are challenging to process and interpret. Here, we describe EPIC ... [more]

Nat. Methods Jul. 15, 2019; (); [Pubmed: 31308550]

Quantitative Score

  • 0.682 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • High confidence protein interactions have an EPIC PPI score >= 0.5

Curated By

  • BioGRID