BAIT

SHU2

YDR078C
Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu1, and promotes error-free DNA repair, Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments
GO Process (4)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

SRS2

HPR5, DNA helicase SRS2, RADH1, RADH, L000000809, L000001578, YJL092W
DNA helicase and DNA-dependent ATPase; involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; functional homolog of human RTEL1
Saccharomyces cerevisiae (S288c)

Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

Publication

Mapping DNA damage-dependent genetic interactions in yeast via party mating and barcode fusion genetics.

Diaz-Mejia JJ, Celaj A, Mellor JC, Cote A, Balint A, Ho B, Bansal P, Shaeri F, Gebbia M, Weile J, Verby M, Karkhanina A, Zhang Y, Wong C, Rich J, Prendergast D, Gupta G, Oeztuerk S, Durocher D, Brown GW, Roth FP

Condition-dependent genetic interactions can reveal functional relationships between genes that are not evident under standard culture conditions. State-of-the-art yeast genetic interaction mapping, which relies on robotic manipulation of arrays of double-mutant strains, does not scale readily to multi-condition studies. Here, we describe barcode fusion genetics to map genetic interactions (BFG-GI), by which double-mutant strains generated via en masse "party" mating ... [more]

Mol. Syst. Biol. Dec. 28, 2017; 14(5);e7985 [Pubmed: 29807908]

Quantitative Score

  • -0.708756851 [Confidence Score]

Throughput

  • High Throughput

Ontology Terms

  • vegetative growth (APO:0000106)

Additional Notes

  • Cultures grown in hydroxyurea (CHEBI:44423)
  • Interactions determined by barcode fusion genetics to map genetic interactions (BFG-GI) using a ZGenetic Interaction Score (GIS)cutoff corresponding to FDR=0.01 and an additional effect?size cutoff (-0.075>GIS>0.075)

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SRS2 SHU2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
-

Curated By

  • BioGRID