BAIT

NSP2AB

nsp2, sars1ab, orf1ab, SARS-CoV nsp2ab, SARS-CoV nsp2, SARS-CoV sars1ab, orf1ab-nsp2, orf1ab-nsp2ab, pp1ab, p65 homolog, R1AB_CVHSA, PRO_0000037310, SARS-CoV-1ab-nsp2ab

Non-structural protein 2; nsp2; p65 homolog

COVID-19 Coronavirus Project

GO Process (0)

GO Function (0)

GO Component (0)

Entrez Gene RefSeq UniprotKB

Severe acute respiratory syndrome coronavirus

PREY

PHB2

BAP, BCAP37, Bap37, PNAS-141, REA, p22

prohibitin 2

GO Process (1)

GO Function (2)

GO Component (6)

Gene Ontology Biological Process

negative regulation of transcription, DNA-templated [IDA]

Gene Ontology Molecular Function

estrogen receptor binding [NAS]
protein binding [IPI]

Gene Ontology Cellular Component

extracellular vesicular exosome [IDA]

mitochondrial inner membrane [ISS]

mitochondrion [IDA]

nuclear matrix [IDA]

nucleus [IDA, ISS]

protein complex [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Severe acute respiratory syndrome coronavirus nonstructural protein 2 interacts with a host protein complex involved in mitochondrial biogenesis and intracellular signaling.

Cornillez-Ty CT, Liao L, Yates JR, Kuhn P, Buchmeier MJ

The severe acute respiratory syndrome coronavirus (SARS-CoV) generates 16 nonstructural proteins (nsp's) through proteolytic cleavage of a large precursor protein. Although several nsp's exhibit catalytic activities that are important for viral replication and transcription, other nsp's have less clearly defined roles during an infection. In order to gain a better understanding of their functions, we attempted to identify host proteins ... [more]

J. Virol. Oct. 01, 2009; 83(19);10314-8 [Pubmed: 19640993]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
NSP2AB PHB2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cornillez-Ty CT (2009)	Low	-	BioGRID	-
NSP2AB PHB2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Stukalov A (2021)	High	3.5225	BioGRID	2754826
NSP2AB PHB2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Stukalov A (2021)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary