Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A Comprehensive Drosophila melanogaster Transcription Factor Interactome.

Shokri L, Inukai S, Hafner A, Weinand K, Hens K, Vedenko A, Gisselbrecht SS, Dainese R, Bischof J, Furger E, Feuz JD, Basler K, Deplancke B, Bulyk ML

Combinatorial interactions among transcription factors (TFs) play essential roles in generating gene expression specificity and diversity in metazoans. Using yeast 2-hybrid (Y2H) assays on nearly all sequence-specific Drosophila TFs, we identified 1,983 protein-protein interactions (PPIs), more than doubling the number of currently known PPIs among Drosophila TFs. For quality assessment, we validated a subset of our interactions using MITOMI and ... [more]

Cell Rep Dec. 16, 2018; 27(3);955-970.e7 [Pubmed: 30995488]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

HR78

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [NAS, TAS]RNA polymerase II regulatory region sequence-specific DNA binding [IDA]ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA, ISS, NAS, TAS]sequence-specific DNA binding transcription factor activity [ISS]

Gene Ontology Cellular Component

CG17806

Two-hybrid

Publication

A Comprehensive Drosophila melanogaster Transcription Factor Interactome.

Throughput

Curated By

DNA binding [NAS, TAS]
RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity [IDA, ISS, NAS, TAS]
sequence-specific DNA binding transcription factor activity [ISS]