BAIT

AKAP8L

HA95, HAP95, NAKAP, NAKAP95, HRIHFB2018
A kinase (PRKA) anchor protein 8-like
GO Process (0)
GO Function (3)
GO Component (4)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

LBR

DHCR14B, LMN2R, PHA, TDRD18, PRO0650
lamin B receptor
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

HA95 is a protein of the chromatin and nuclear matrix regulating nuclear envelope dynamics.

Martins SB, Eide T, Steen RL, Jahnsen T, Skalhegg B S, Collas P

We report a role for HA95, a nuclear protein with high homology to the nuclear A-kinase anchoring protein AKAP95, in the regulation of nuclear envelope-chromatin interactions. Biochemical and photobleaching data indicate that HA95 is tightly associated with chromatin and the nuclear matrix/lamina network in interphase, and bound to chromatin at mitosis. HA95 resides in a complex together with lamin B ... [more]

J. Cell. Sci. Nov. 01, 2000; 113(0);3703-13 [Pubmed: 11034899]

Throughput

  • Low Throughput

Curated By

  • BioGRID