BAIT
VPS33B
vacuolar protein sorting 33 homolog B (yeast)
GO Process (6)
GO Function (1)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
DHX36
DDX36, G4R1, MLEL1, RHAU
DEAH (Asp-Glu-Ala-His) box polypeptide 36
GO Process (8)
GO Function (10)
GO Component (4)
Gene Ontology Biological Process
- ATP catabolic process [IBA, IDA]
- RNA processing [IBA]
- RNA secondary structure unwinding [IDA]
- innate immune response [TAS]
- ossification [ISS]
- positive regulation of telomere maintenance [IMP]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of type I interferon production [TAS]
Gene Ontology Molecular Function- ATP-dependent RNA helicase activity [IBA]
- DNA-dependent ATPase activity [IDA]
- G-quadruplex DNA binding [IDA]
- G-quadruplex RNA binding [IMP]
- core promoter binding [IDA]
- double-stranded RNA binding [IDA]
- histone deacetylase binding [ISS]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- transcription regulatory region DNA binding [ISS]
- ATP-dependent RNA helicase activity [IBA]
- DNA-dependent ATPase activity [IDA]
- G-quadruplex DNA binding [IDA]
- G-quadruplex RNA binding [IMP]
- core promoter binding [IDA]
- double-stranded RNA binding [IDA]
- histone deacetylase binding [ISS]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- transcription regulatory region DNA binding [ISS]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.
Multi-subunit tethering complexes control membrane fusion events in eukaryotic cells. Class C core vacuole/endosome tethering (CORVET) and homotypic fusion and vacuole protein sorting (HOPS) are two such complexes, both containing the Sec1/Munc18 protein subunit VPS33A. Metazoans additionally possess VPS33B, which has considerable sequence similarity to VPS33A but does not integrate into CORVET or HOPS complexes and instead stably interacts with ... [more]
J Mol Biol Dec. 06, 2017; 430(14);2153-2163 [Pubmed: 29778605]
Throughput
- High Throughput
Additional Notes
- Interacting proteins identified by BioID with a Bayesian false discovery rate BFDR of =< 0.01
- Proximity-dependent biotinylation (BioID) assay
- Wild type bait
Curated By
- BioGRID