BAIT
E
env, envelope, MERS-CoV E, E protein, emp, MERS-CoV E, VEMP_CVEMC, PRO_0000422468, G128_gp07
Envelope small membrane protein
GO Process (0)
GO Function (0)
GO Component (0)
Middle-East Respiratory Syndrome-related Coronavirus
PREY
INADL
Cipp, InaD-like, PATJ, hINADL, RP4-537K17.1
InaD-like (Drosophila)
GO Process (4)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Protein-peptide
An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.
Publication
Host PDZ-containing proteins targeted by SARS-CoV-2.
Small linear motifs targeting protein interacting domains called PSD-95/Dlg/ZO-1 (PDZ) have been identified at the C terminus of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins E, 3a, and N. Using a high-throughput approach of affinity-profiling against the full human PDZome, we identified sixteen human PDZ binders of SARS-CoV-2 proteins E, 3A, and N showing significant interactions with dissociation ... [more]
FEBS J Dec. 01, 2020; 288(17);5148-5162 [Pubmed: 33864728]
Throughput
- High Throughput
Additional Notes
- A peptide containing the PBM sequence of the viral E protein was used as a bait to screen a human PDZ domain library in an in vitro holdup assay.
- Significant PDZ/PBM interaction pairs had a binding intensity BI > 0.2 (~ 100 um dissociation constant).
Curated By
- BioGRID