GABPA
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription coactivator activity [TAS]
- transcription regulatory region DNA binding [IDA]
- DNA binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IDA]
- sequence-specific DNA binding transcription factor activity [TAS]
- transcription coactivator activity [TAS]
- transcription regulatory region DNA binding [IDA]
Gene Ontology Cellular Component
MED1
Gene Ontology Biological Process
- ERK1 and ERK2 cascade [IDA]
- androgen biosynthetic process [IMP]
- androgen receptor signaling pathway [IDA]
- angiogenesis [ISS]
- cell morphogenesis [IMP]
- cellular lipid metabolic process [TAS]
- cellular response to epidermal growth factor stimulus [IDA]
- cellular response to steroid hormone stimulus [IDA]
- cellular response to thyroid hormone stimulus [IDA]
- erythrocyte development [ISS]
- fat cell differentiation [IDA]
- gene expression [TAS]
- intracellular steroid hormone receptor signaling pathway [IDA]
- keratinocyte differentiation [IMP]
- lens development in camera-type eye [ISS]
- mRNA transcription from RNA polymerase II promoter [ISS]
- megakaryocyte development [ISS]
- negative regulation of apoptotic process [ISS]
- negative regulation of keratinocyte proliferation [IMP]
- negative regulation of neuron differentiation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of gene expression [IDA, IMP]
- positive regulation of keratinocyte differentiation [IMP]
- positive regulation of receptor activity [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [IDA]
- regulation of RNA biosynthetic process [IMP]
- regulation of cell cycle [NAS]
- regulation of transcription from RNA polymerase I promoter [IDA]
- small molecule metabolic process [TAS]
- thyroid hormone mediated signaling pathway [IMP]
- transcription initiation from RNA polymerase II promoter [IDA, TAS]
Gene Ontology Molecular Function- LBD domain binding [IPI]
- RNA polymerase II transcription cofactor activity [IDA]
- chromatin binding [IMP]
- core promoter binding [IDA]
- estrogen receptor binding [IPI]
- ligand-dependent nuclear receptor binding [IDA, IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IMP, NAS]
- mediator complex binding [IDA]
- nuclear hormone receptor binding [IPI]
- peroxisome proliferator activated receptor binding [IPI]
- protein binding [IPI]
- receptor activity [IDA]
- retinoic acid receptor binding [IPI]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [ISS]
- thyroid hormone receptor binding [IDA, IPI]
- thyroid hormone receptor coactivator activity [IMP]
- transcription coactivator activity [IDA, IMP]
- transcription cofactor activity [IDA]
- transcription factor binding [IPI]
- vitamin D receptor binding [IPI, NAS, TAS]
- LBD domain binding [IPI]
- RNA polymerase II transcription cofactor activity [IDA]
- chromatin binding [IMP]
- core promoter binding [IDA]
- estrogen receptor binding [IPI]
- ligand-dependent nuclear receptor binding [IDA, IPI]
- ligand-dependent nuclear receptor transcription coactivator activity [IMP, NAS]
- mediator complex binding [IDA]
- nuclear hormone receptor binding [IPI]
- peroxisome proliferator activated receptor binding [IPI]
- protein binding [IPI]
- receptor activity [IDA]
- retinoic acid receptor binding [IPI]
- sequence-specific DNA binding RNA polymerase II transcription factor activity [ISS]
- thyroid hormone receptor binding [IDA, IPI]
- thyroid hormone receptor coactivator activity [IMP]
- transcription coactivator activity [IDA, IMP]
- transcription cofactor activity [IDA]
- transcription factor binding [IPI]
- vitamin D receptor binding [IPI, NAS, TAS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Regulation of Aurora-A kinase gene expression via GABP recruitment of TRAP220/MED1.
The TRAP/Mediator coactivator complex serves as a functional interface between DNA-bound transactivators and the RNA polymerase II-associated basal transcription apparatus. TRAP220/MED1 is a variably associated subunit of the complex that plays a specialized role in selectively targeting TRAP/Mediator to specific genes. Ablation of the Trap220/Med1 gene in mice impairs embryonic cell growth, yet the underlying mechanism is unknown. In this ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| MED1 GABPA | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| GABPA MED1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID