BAIT

MF

BcDNA:GH23305, CG6803, DmMf, Dmel\CG6803, Zeelin1, z1, Dmel_CG6803
Myofilin
GO Process (3)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Drosophila melanogaster
PREY

LASP

CG3849, Dmel\CG3849, anon-73Bd, cDNA B, Dmel_CG3849
CG3849 gene product from transcript CG3849-RB
Drosophila melanogaster

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Next-generation large-scale binary protein interaction network for Drosophila melanogaster.

Tang HW, Spirohn K, Hu Y, Hao T, Kovacs IA, Gao Y, Binari R, Yang-Zhou D, Wan KH, Bader JS, Balcha D, Bian W, Booth BW, Cote AG, de Rouck S, Desbuleux A, Goh KY, Kim DK, Knapp JJ, Lee WX, Lemmens I, Li C, Li M, Li R, Lim HJ, Liu Y, Luck K, Markey D, Pollis C, Rangarajan S, Rodiger J, Schlabach S, Shen Y, Sheykhkarimli D, TeeKing B, Roth FP, Tavernier J, Calderwood MA, Hill DE, Celniker SE, Vidal M, Perrimon N, Mohr SE

Generating reference maps of interactome networks illuminates genetic studies by providing a protein-centric approach to finding new components of existing pathways, complexes, and processes. We apply state-of-the-art methods to identify binary protein-protein interactions (PPIs) for Drosophila melanogaster. Four all-by-all yeast two-hybrid (Y2H) screens of?>?10,000 Drosophila proteins result in the 'FlyBi' dataset of 8723 PPIs among 2939 proteins. Testing subsets of ... [more]

Nat Commun Apr. 15, 2023; 14(1);2162 [Pubmed: 37061542]

Throughput

  • High Throughput

Additional Notes

  • interaction identified by large-scale Y2H screening and validated by pairwise testing

Curated By

  • BioGRID