BAIT

ORF3A

SARS-CoV2 ORF3a, SARS-CoV-2 ORF3a, Protein 3a, 3a, AP3A_SARS2, PRO_0000449650, GU280_gp03
Protein 3a
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

SHMT2

GLYA, HEL-S-51e, SHMT
serine hydroxymethyltransferase 2 (mitochondrial)
GO Process (1)
GO Function (2)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Some aspects of the life of SARS-CoV-2 ORF3a protein in mammalian cells.

Jiao S, Miranda P, Li Y, Maric D, Holmgren M

The accessory protein ORF3a, from SARS-CoV-2, plays a critical role in viral infection and pathogenesis. Here, we characterized ORF3a assembly, ion channel activity, subcellular localization, and interactome. At the plasma membrane, ORF3a exists mostly as monomers and dimers, which do not alter the native cell membrane conductance, suggesting that ORF3a does not function as a viroporin at the cell surface. ... [more]

Heliyon Aug. 01, 2023; 9(8);e18754 [Pubmed: 37609425]

Throughput

  • High Throughput

Additional Notes

  • Proximity label MS was carried out to identify high confidence protein interactors with a p-value < 0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ORF3A SHMT2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
3501405

Curated By

  • BioGRID