BAIT

ORF3A

SARS-CoV2 ORF3a, SARS-CoV-2 ORF3a, Protein 3a, 3a, AP3A_SARS2, PRO_0000449650, GU280_gp03
Protein 3a
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

FLG2

IFPS, RP1-14N1.3
filaggrin family member 2
Glioblastoma Project
GO Process (1)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Some aspects of the life of SARS-CoV-2 ORF3a protein in mammalian cells.

Jiao S, Miranda P, Li Y, Maric D, Holmgren M

The accessory protein ORF3a, from SARS-CoV-2, plays a critical role in viral infection and pathogenesis. Here, we characterized ORF3a assembly, ion channel activity, subcellular localization, and interactome. At the plasma membrane, ORF3a exists mostly as monomers and dimers, which do not alter the native cell membrane conductance, suggesting that ORF3a does not function as a viroporin at the cell surface. ... [more]

Heliyon Aug. 01, 2023; 9(8);e18754 [Pubmed: 37609425]

Throughput

  • High Throughput

Additional Notes

  • Proximity label MS was carried out to identify high confidence protein interactors with a p-value < 0.05

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ORF3A FLG2
Co-fractionation
Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

High-BioGRID
3805136

Curated By

  • BioGRID