BAIT

E7

HpV9gp2
early protein
GO Process (0)
GO Function (0)
GO Component (0)
Human papillomavirus (9)
PREY

PUM1

HSPUM, PUMH, PUMH1, PUML1, RP1-65J11.4
pumilio RNA-binding family member 1
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Comparative analysis of virus-host interactomes with a mammalian high-throughput protein complementation assay based on Gaussia princeps luciferase.

Neveu G, Cassonnet P, Vidalain PO, Rolloy C, Mendoza J, Jones L, Tangy F, Muller M, Demeret C, Tafforeau L, Lotteau V, Rabourdin-Combe C, Trave G, Dricot A, Hill DE, Vidal M, Favre M, Jacob Y

Comparative interactomics is a strategy for inferring potential interactions among orthologous proteins or "interologs". Herein we focus, in contrast to standard homology-based inference, on the divergence of protein interaction profiles among closely related organisms, showing that the approach can correlate specific traits to phenotypic differences. As a model, this new comparative interactomic approach was applied at a large scale to ... [more]

Methods Dec. 01, 2012; 58(4);349-59 [Pubmed: 22898364]

Throughput

  • High Throughput

Curated By

  • BioGRID