PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

Comparative analysis of virus-host interactomes with a mammalian high-throughput protein complementation assay based on Gaussia princeps luciferase.

Neveu G, Cassonnet P, Vidalain PO, Rolloy C, Mendoza J, Jones L, Tangy F, Muller M, Demeret C, Tafforeau L, Lotteau V, Rabourdin-Combe C, Trave G, Dricot A, Hill DE, Vidal M, Favre M, Jacob Y

Comparative interactomics is a strategy for inferring potential interactions among orthologous proteins or "interologs". Herein we focus, in contrast to standard homology-based inference, on the divergence of protein interaction profiles among closely related organisms, showing that the approach can correlate specific traits to phenotypic differences. As a model, this new comparative interactomic approach was applied at a large scale to ... [more]

Methods Dec. 01, 2012; 58(4);349-59 [Pubmed: 22898364]

Throughput

  • High Throughput

Ontology Terms

  • hek-293t cell (BTO:0002181) [embryonic kidney cell line (BTO:0002733)]
  • kidney epithelial cell (CL:0002518)
  • kidney (BTO:0000671)

Additional Notes

  • Gaussia princeps luciferase Complementation Assay

Curated By

  • BioGRID