BAIT

NSP6

ORF1ab, ORF1ab-nsp6, SARS-CoV2 nsp6, SARS-CoV-2 nsp6, R1AB_SARS2, PRO_0000449624, GU280_gp01_nsp6
Non-structural protein 6
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

ACTR1B

ARP1B, CTRN2, PC3
ARP1 actin-related protein 1 homolog B, centractin beta (yeast)
GO Process (1)
GO Function (1)
GO Component (6)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human protein interaction networks of ancestral and variant SARS-CoV-2 in organ-specific cells and bodily fluids.

Broderick K, Moutaoufik MT, Saccon T, Malty R, Amin S, Phanse S, Joseph TP, Zilocchi M, Hosseinnia A, Istace Z, Hajikarimlou M, Abrar S, Fisher J, Brassard R, Perera R, Kumar A, Aoki H, Rahmatbakhsh M, Jessulat M, Kobasa D, Dehne F, Prasad B, Gagarinova A, Joanne Lemieux M, Cochrane A, Houry WA, Aly KA, Golshani A, Babu M

Understanding SARS-CoV-2 human protein-protein interactions (PPIs) and the host response to infection is essential for developing effective COVID-19 antivirals. However, how the ancestral virus and its variants remodel virus-host protein assemblies in various organ-specific cells and bodily fluids remains unclear. Here, we conduct 639 affinity-purifications by tagging and expressing 28 SARS-CoV-2 and spike proteins from the ancestral virus and four ... [more]

Nat Commun Jul. 01, 2025; 16(1);5784 [Pubmed: 40593736]

Throughput

  • High Throughput

Ontology Terms

  • lung endothelial cell (CL:1001567) [lung (BTO:0000763)]
  • hek-293 cell (BTO:0000007) [kidney (BTO:0000671)]

Additional Notes

  • RF score threshold > 0.8

Curated By

  • BioGRID