BAIT

PIP82

CG11219, Dmel\CG11219, Dmel_CG11219
CG11219 gene product from transcript CG11219-RA
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Drosophila melanogaster
PREY

CPR66D

CG32029, CG6458, CG6469, DmelCpr66D, Dmel\CG32029, Dmel_CG32029
Cuticular protein 66D
GO Process (1)
GO Function (2)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Drosophila melanogaster

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Proximity labeling reveals interactions necessary to maintain the distinct apical domains of Drosophila photoreceptors.

Sastry L, Rylee J, Mahato S, Zelhof AC

Specialized membrane and cortical protein regions are common features of cells and are utilized to isolate differential cellular functions. In Drosophila photoreceptors, the apical membrane domain is defined by two distinct morphological membranes: the rhabdomere microvilli and the stalk membrane. To define the apical cortical protein complexes, we performed proximity labeling screens utilizing the rhabdomeric-specific protein PIP82 as bait. We ... [more]

J Cell Sci Dec. 01, 2024; 137(23); [Pubmed: 39540276]

Throughput

  • High Throughput

Additional Notes

  • Hit in Adult fly retina: 4 fold over control
  • Proximity label MS was carried out to identify high confidence protein interactors in adult fly retinas that are enriched greater than or equal 3 fold over control

Curated By

  • BioGRID