BAIT
PIP82
CG11219, Dmel\CG11219, Dmel_CG11219
CG11219 gene product from transcript CG11219-RA
GO Process (1)
GO Function (0)
GO Component (0)
Gene Ontology Biological Process
Drosophila melanogaster
PREY
ALPHA-SPEC
3A9, CG1977, Dmel\CG1977, FBtr0072789, FCP-B, Spec, Spectrin, alfa-Spec, alpha, alpha-Sp, alpha-Spc, alpha-Spe, alpha-Spect, alpha-Spectrin, alphaSp, alphaSpec, alphaSpectrin, anon-EST:fe2E2, dre3, klo, l(3)04276, l(3)62Bd, l(3)alpha-Spec, l(3)dre3, Dmel_CG1977
alpha Spectrin
GO Process (21)
GO Function (5)
GO Component (10)
Gene Ontology Biological Process
- axon midline choice point recognition [IGI]
- central nervous system development [IMP]
- cytoskeleton organization [ISS]
- epithelium development [IMP]
- female germline ring canal formation, actin assembly [TAS]
- fusome organization [TAS]
- germ-line cyst formation [TAS]
- germarium-derived female germ-line cyst formation [IMP]
- germarium-derived oocyte fate determination [IMP, TAS]
- instar larval development [NAS]
- long-term strengthening of neuromuscular junction [IMP]
- maintenance of presynaptic active zone structure [IDA]
- negative regulation of microtubule depolymerization [IMP]
- neuromuscular synaptic transmission [IMP]
- oocyte construction [IMP]
- ovarian follicle cell development [TAS]
- plasma membrane organization [TAS]
- regulation of cell shape [NAS]
- regulation of synapse organization [IMP]
- single organismal cell-cell adhesion [NAS]
- spectrosome organization [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Drosophila melanogaster
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Proximity labeling reveals interactions necessary to maintain the distinct apical domains of Drosophila photoreceptors.
Specialized membrane and cortical protein regions are common features of cells and are utilized to isolate differential cellular functions. In Drosophila photoreceptors, the apical membrane domain is defined by two distinct morphological membranes: the rhabdomere microvilli and the stalk membrane. To define the apical cortical protein complexes, we performed proximity labeling screens utilizing the rhabdomeric-specific protein PIP82 as bait. We ... [more]
J Cell Sci Dec. 01, 2024; 137(23); [Pubmed: 39540276]
Throughput
- High Throughput
Additional Notes
- Hit in S2 cells: SAINT P = 1
- Proximity label MS was carried out to identify high confidence protein interactors in S2 cells with a SAINT P of greater than or equal to 0.8
Curated By
- BioGRID