BAIT

PIP82

CG11219, Dmel\CG11219, Dmel_CG11219
CG11219 gene product from transcript CG11219-RA
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Drosophila melanogaster
PREY

POD1

BEST:LD37601, BcDNA:LD08951, CG4532, Dmel\CG4532, Dpod1, POD-1, coronin, dmPod, Dmel_CG4532
CG4532 gene product from transcript CG4532-RG
Drosophila melanogaster

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Proximity labeling reveals interactions necessary to maintain the distinct apical domains of Drosophila photoreceptors.

Sastry L, Rylee J, Mahato S, Zelhof AC

Specialized membrane and cortical protein regions are common features of cells and are utilized to isolate differential cellular functions. In Drosophila photoreceptors, the apical membrane domain is defined by two distinct morphological membranes: the rhabdomere microvilli and the stalk membrane. To define the apical cortical protein complexes, we performed proximity labeling screens utilizing the rhabdomeric-specific protein PIP82 as bait. We ... [more]

J Cell Sci Dec. 01, 2024; 137(23); [Pubmed: 39540276]

Throughput

  • High Throughput

Additional Notes

  • Hit in S2 cells: SAINT P = 0.86
  • Proximity label MS was carried out to identify high confidence protein interactors in S2 cells with a SAINT P of greater than or equal to 0.8

Curated By

  • BioGRID