BAIT

AWD

1084/08, BcDNA:GM19775, BcDNA:RH27794, CG2210, Dmel\CG2210, K-pn, Kpn, NDKB, NDPK, Nm23/awd, anon-EST:Liang-2.27, anon-EST:Liang-2.28, anon-WO0172774.80, anon-WO0172774.82, clone 2.27, clone 2.28, e(shi)A, eshiA, l(3)L8700, l(3)j2A4, Dmel_CG2210
abnormal wing discs
Drosophila melanogaster
PREY

CG30339

BcDNA:RE54230, Dmel\CG30339, Dmel_CG30339
CG30339 gene product from transcript CG30339-RA
GO Process (0)
GO Function (0)
GO Component (0)
Drosophila melanogaster

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

A protein complex network of Drosophila melanogaster.

Guruharsha KG, Rual JF, Zhai B, Mintseris J, Vaidya P, Vaidya N, Beekman C, Wong C, Rhee DY, Cenaj O, McKillip E, Shah S, Stapleton M, Wan KH, Yu C, Parsa B, Carlson JW, Chen X, Kapadia B, VijayRaghavan K, Gygi SP, Celniker SE, Obar RA, Artavanis-Tsakonas S

Determining the composition of protein complexes is an essential step toward understanding the cell as an integrated system. Using coaffinity purification coupled to mass spectrometry analysis, we examined protein associations involving nearly 5,000 individual, FLAG-HA epitope-tagged Drosophila proteins. Stringent analysis of these data, based on a statistical framework designed to define individual protein-protein interactions, led to the generation of a ... [more]

Cell Oct. 28, 2011; 147(3);690-703 [Pubmed: 22036573]

Throughput

  • High Throughput

Curated By

  • BioGRID