An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Complex of FAF1 with VCP-Npl4-Ufd1 and polyubiquitinated proteins promotes endoplasmic reticulum-associated degradation (ERAD).

Lee JJ, Park J, Jeong J, Jeon H, Yoon JB, Kim EE, Lee KJ

Fas-associated factor 1 (FAF1) is an ubiquitin receptor containing multiple ubiquitin related domains including UBA, UBL1, UBL2, and UBX. We previously showed that N-terminal UBA domain recognizes K48-ubiquitin linkage to recruit polyubiquitinated proteins, and that a C-terminal UBX domain interacts with valosin-containing protein (VCP). This study shows that FAF1 interacts only with VCP complexed with Npl4-Ufd1 heterodimer, a requirement for ... [more]

J. Biol. Chem. Jan. 04, 2013; 0(0); [Pubmed: 23293021]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
FAF1 NPLOC4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Alexandru G (2008)	Low	-	BioGRID	-
NPLOC4 FAF1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Raman M (2015)	High	-	BioGRID	-
FAF1 NPLOC4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Raman M (2015)	High	-	BioGRID	-
FAF1 NPLOC4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Song S (2022)	Low	-	BioGRID	-
FAF1 NPLOC4	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Xie F (2017)	High	-	BioGRID	-
FAF1 NPLOC4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Alexandru G (2008)	Low	-	BioGRID	-
FAF1 NPLOC4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Haenzelmann P (2011)	Low	-	BioGRID	-
FAF1 NPLOC4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Lee JJ (2013)	Low	-	BioGRID	-
FAF1 NPLOC4	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	Xie F (2017)	Low	-	BioGRID	-
FAF1 NPLOC4	Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.	Xie F (2017)	Low	-	BioGRID	2594544

Curated By

BioGRID

Interaction Summary

FAF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NF-kappaB binding [IPI]heat shock protein binding [IDA]protein binding [IPI]protein kinase binding [IDA]protein kinase regulator activity [NAS]ubiquitin binding [IDA]ubiquitin protein ligase binding [IDA]

Gene Ontology Cellular Component

NPLOC4