BAIT
NOP1
CaO19.3138, C4_06720W, orf19.3138, IPF3267.2, C4_06720W_B, CaO19.10650, CaO6.5875, Contig4-3009_0002, IPF24186.1, CA3570, orf19.10650, orf6.5875, CAWG_03157, C4_06720W_A
Nucleolar protein; flucytosine induced; Hap43-induced; Spider biofilm repressed
GO Process (0)
GO Function (0)
GO Component (0)
Candida albicans (SC5314)
PREY
SIK1
Contig4-3105_0047, IPF929.2, CaO6.8917, orf19.7569, CR_09950C, CA5975, CAWG_02259, CaO19.7569, CR_09950C_B, orf6.8917, CR_09950C_A
Putative U3 snoRNP protein; Hap43p-induced gene; physically interacts with TAP-tagged Nop1p
GO Process (0)
GO Function (0)
GO Component (0)
Candida albicans (SC5314)
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
A toolbox for epitope-tagging and genome-wide location analysis in Candida albicans.
BACKGROUND: Candida albicans is a diploid pathogenic fungus not yet amenable to routine genetic investigations. Understanding aspects of the regulation of its biological functions and the assembly of its protein complexes would lead to further insight into the biology of this common disease-causing microbial agent. RESULTS: We have developed a toolbox allowing in vivo protein tagging by PCR-mediated homologous recombination ... [more]
BMC Genomics Dec. 06, 2008; 9(0);578 [Pubmed: 19055720]
Throughput
- Low Throughput
Curated By
- BioGRID