BAIT

ULP2

SPAC17A5.07c
SUMO deconjugating cysteine peptidase Ulp2 (predicted)
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)
PREY

RPA2

SPBP23A10.07
DNA-directed RNA polymerase I complex subunit Rpa2
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Schizosaccharomyces pombe (972h)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The S. pombe Translation Initiation Factor eIF4G Is Sumoylated and Associates with the SUMO Protease Ulp2.

Jongjitwimol J, Feng M, Zhou L, Wilkinson O, Small L, Baldock R, Taylor DL, Smith D, Bowler LD, Morley SJ, Watts FZ

SUMO is a small post-translational modifier, that is attached to lysine residues in target proteins. It acts by altering protein-protein interactions, protein localisation and protein activity. SUMO chains can also act as substrates for ubiquitination, resulting in proteasome-mediated degradation of the target protein. SUMO is removed from target proteins by one of a number of specific proteases. The processes of ... [more]

PLoS ONE May. 14, 2014; 9(5);e94182 [Pubmed: 24818994]

Throughput

  • Low Throughput

Additional Notes

  • figure 3, table 2/S1.

Curated By

  • BioGRID