Rab11-FIP2, an adaptor protein connecting cellular components involved in internalization and recycling of epidermal growth factor receptors.

Rab11-FIP2 is a member of a newly identified family of Rab11-binding proteins that have been implicated in the function of recycling endosomes. Here we show that Rab11-FIP2 may also be involved with the process of receptor-mediated endocytosis. First we demonstrate that Rab11-FIP2 contains an NPF motif that allows it to ...
bind Reps1, a member of a family of EH domain proteins involved in endocytosis. We also show that Rab11-FIP2 associates with the alpha-adaptin subunit of AP-2 complexes, which are known to recruit receptors into clathrin-coated vesicles. Finally, we find that overexpression of Rab11-FIP2 suppresses the internalization of epidermal growth factor receptors, but not transferrin receptors, through binding sites that promote complex formation with Rab11, Reps1, and alpha-adaptin. These findings suggest that Rab11-FIP2 may participate in the coupling of receptor-mediated endocytosis to the subsequent sorting of receptor-containing vesicles in endosomes.
Mesh Terms:
3T3 Cells, Adaptor Protein Complex 2, Adaptor Protein Complex alpha Subunits, Amino Acid Motifs, Amino Acid Sequence, Animals, Binding Sites, COS Cells, Carrier Proteins, Endocytosis, Endosomes, Glutathione Transferase, Membrane Proteins, Mice, Models, Biological, Molecular Sequence Data, Peptides, Plasmids, Precipitin Tests, Protein Binding, Protein Structure, Tertiary, Receptor, Epidermal Growth Factor, Sequence Homology, Amino Acid, Software, Spectrometry, Fluorescence, Transfection, Two-Hybrid System Techniques, rab GTP-Binding Proteins
J. Biol. Chem.
Date: Dec. 20, 2002
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