The SWI/SNF chromatin remodeling complex regulates myocardin-induced smooth muscle-specific gene expression.
OBJECTIVE: Regulatory complexes comprising myocardin and serum response factor (SRF) are critical for the transcriptional regulation of many smooth muscle-specific genes. However, little is known about the epigenetic mechanisms that regulate the activity of these complexes. In the current study, we investigated the role of SWI/SNF ATP-dependent chromatin remodeling enzymes ... in regulating the myogenic activity of myocardin. METHODS AND RESULTS: We found that both Brg1 and Brm are required for maintaining expression of several smooth muscle-specific genes in primary cultures of aortic smooth muscle cells. Furthermore, the ability of myocardin to induce expression of smooth muscle-specific genes is abrogated in cells expressing dominant negative Brg1. In SW13 cells, which lack endogenous Brg1 and Brm1, myocardin is unable to induce expression of smooth muscle-specific genes. Whereas, reconstitution of wild-type, or bromodomain mutant forms Brg1 or Brm1, into SW13 cells restored their responsiveness to myocardin. SWI/SNF complexes were found to be required for myocardin to increase SRF binding to the promoters of smooth muscle-specific genes. Brg1 and Brm directly bind to the N terminus of myocardin, in vitro, through their ATPase domains and Brg1 forms a complex with SRF and myocardin in vivo in smooth muscle cells. CONCLUSIONS: These data demonstrate that the ability of myocardin to induce smooth muscle-specific gene expression is dependent on its interaction with SWI/SNF ATP-dependent chromatin remodeling complexes.
Mesh Terms:
Animals, Cell Differentiation, Cells, Cultured, Chromatin Assembly and Disassembly, DNA Helicases, Gene Expression Regulation, Humans, Mice, Muscle Development, Muscle, Smooth, Vascular, Mutation, Nuclear Proteins, Promoter Regions, Genetic, Protein Binding, Protein Structure, Tertiary, RNA Interference, RNA, Small Interfering, Serum Response Factor, Trans-Activators, Transcription Factors, Transfection
Animals, Cell Differentiation, Cells, Cultured, Chromatin Assembly and Disassembly, DNA Helicases, Gene Expression Regulation, Humans, Mice, Muscle Development, Muscle, Smooth, Vascular, Mutation, Nuclear Proteins, Promoter Regions, Genetic, Protein Binding, Protein Structure, Tertiary, RNA Interference, RNA, Small Interfering, Serum Response Factor, Trans-Activators, Transcription Factors, Transfection
Arterioscler. Thromb. Vasc. Biol.
Date: Jun. 01, 2009
PubMed ID: 19342595
View in: Pubmed Google Scholar
Download Curated Data For This Publication
100539
Switch View:
- Interactions 7