Identification of 14-3-3epsilon substrates from embryonic murine brain.
Mice deficient in 14-3-3epsilon exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3epsilon interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine ... 680 was found essential for its interaction with 14-3-3epsilon and for maintaining USP8 in the cytosol.
Mesh Terms:
14-3-3 Proteins, Amino Acid Sequence, Animals, Binding Sites, Brain, COS Cells, Cercopithecus aethiops, Endopeptidases, Endosomal Sorting Complexes Required for Transport, Mass Spectrometry, Mice, Microscopy, Fluorescence, Molecular Sequence Data, Phosphorylation, Ubiquitin Thiolesterase
14-3-3 Proteins, Amino Acid Sequence, Animals, Binding Sites, Brain, COS Cells, Cercopithecus aethiops, Endopeptidases, Endosomal Sorting Complexes Required for Transport, Mass Spectrometry, Mice, Microscopy, Fluorescence, Molecular Sequence Data, Phosphorylation, Ubiquitin Thiolesterase
J. Proteome Res.
Date: Sep. 01, 2006
PubMed ID: 16944949
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