Interaction of plexin-B1 with PDZ domain-containing Rho guanine nucleotide exchange factors.
The Rho family GTPase has been implicated in plexin-B1, a receptor for Semaphorin 4D (Sema4D), mediating signal transduction. Rho may also play a function in this signaling pathway as well as Rac, but the mechanisms for Rho regulation are poorly understood. In this study, we have identified two kinds of ... PDZ domain-containing Rho-specific guanine nucleotide exchange factors (RhoGEFs) as proteins interacting with plexin-B1 cytoplasmic domain. These PDZ domain-containing RhoGEFs showed significant homology to human KIAA0380 (PDZ-RhoGEF) and LARG (KIAA0382), respectively. Both KIAA0380 and LARG could bind plexin-B1 and a deletion mutant analysis of plexin-B1, KIAA0380 and LARG revealed that KIAA0380 and LARG bound plexin-B1 cytoplasmic tail through their PDZ domains. The tissue distribution analysis indicated that plexin-B1 was co-localized with KIAA0380 and LARG in various tissues. Immunocytochemical analysis showed that LARG was recruited to plasma membrane by plexin-B1. These results suggest that PDZ domain-containing RhoGEFs play a role in Sema4D-plexin-B1 mediating signal transduction.
Mesh Terms:
Animals, Binding Sites, Cells, Cultured, Gene Expression, Guanine Nucleotide Exchange Factors, Humans, Nerve Tissue Proteins, Protein Structure, Tertiary, Rabbits, Receptors, Cell Surface, Transfection
Animals, Binding Sites, Cells, Cultured, Gene Expression, Guanine Nucleotide Exchange Factors, Humans, Nerve Tissue Proteins, Protein Structure, Tertiary, Rabbits, Receptors, Cell Surface, Transfection
Biochem. Biophys. Res. Commun.
Date: Sep. 13, 2002
PubMed ID: 12220504
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