The plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding.
The small GTPase Rac has been implicated in growth cone guidance mediated by semaphorins and their receptors. Here we demonstrate that plexin-B1, a receptor for Semaphorin4D (Sema4D), and p21-activated kinase (PAK) can compete for the interaction with active Rac and plexin-B1 can inhibit Rac-induced PAK activation. We have also demonstrated ... that expression of active Rac enhances the ability of plexin-B1 to interact with Sema4D. Active Rac stimulates the localization of plexin-B1 to the cell surface. The enhancement in Sema4D binding depends on the ability of Rac to bind plexin-B1. These observations support a model where signaling between Rac and plexin-B1 is bidirectional; Rac modulates plexin-B1 activity and plexin-B1 modulates Rac function.
Mesh Terms:
Animals, Antigens, CD, Biotinylation, COS Cells, Cell Line, Cell Membrane, Dose-Response Relationship, Drug, Endopeptidase K, Enzyme Activation, Glycoproteins, Humans, Kinetics, Ligands, Membrane Glycoproteins, Models, Biological, Nerve Tissue Proteins, Plasmids, Protein Binding, Protein Structure, Tertiary, Protein-Serine-Threonine Kinases, Proteins, Receptors, Cell Surface, Semaphorin-3A, Semaphorins, Signal Transduction, p21-Activated Kinases, rac GTP-Binding Proteins
Animals, Antigens, CD, Biotinylation, COS Cells, Cell Line, Cell Membrane, Dose-Response Relationship, Drug, Endopeptidase K, Enzyme Activation, Glycoproteins, Humans, Kinetics, Ligands, Membrane Glycoproteins, Models, Biological, Nerve Tissue Proteins, Plasmids, Protein Binding, Protein Structure, Tertiary, Protein-Serine-Threonine Kinases, Proteins, Receptors, Cell Surface, Semaphorin-3A, Semaphorins, Signal Transduction, p21-Activated Kinases, rac GTP-Binding Proteins
Genes Dev.
Date: Apr. 01, 2002
PubMed ID: 11937491
View in: Pubmed Google Scholar
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