Secretion is required for late events in the cell-fusion pathway of mating yeast.

Secretory vesicles accumulate adjacent to the contact site between the two cells of a yeast mating pair before they fuse, but there is no direct evidence that secretion is required to complete fusion. In this study, temperature-sensitive secretion (sec(ts)) mutants were used to investigate the role of secretion in yeast ...
cell fusion. Cell fusion arrested less than 5 minutes after inhibiting secretion. This rapid fusion arrest was not an indirect consequence of reduced mating pheromone signaling, mating-pair assembly or actin polarity. Furthermore, secretion was required to complete cell fusion when it was transiently inhibited by addition and removal of the lipophilic styryl dye, FM4-64. These results indicate that ongoing secretion is required for late events in the cell-fusion pathway, which include plasma-membrane fusion and the completion of cell-wall remodeling, and they demonstrate a just-in-time delivery mechanism for the cell-fusion machinery.
Mesh Terms:
Cytochrome-c Peroxidase, Cytochromes c, Kinetics, Models, Molecular, Mutation, Osmolar Concentration, Oxidation-Reduction, Protein Binding, Protein Structure, Quaternary, Recombinant Proteins, Saccharomyces cerevisiae
J. Cell. Sci.
Date: Jun. 01, 2010
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